BCH 5425 Molecular Biology and Biotechnology

Spring 1999 NAME:________________

Wednesday Mar 3

1. Name the following structures, molecules or areas from a bacterial cell (15 points). Also: what type of bacteria is this from?

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2. A particular peritrichous bacterium has six flagella which rotate 73% of the time counterclockwise. What proportion of the time is this bacterium tumbling? (5 points)

3. What are the five features of a bacteria which is chemotrophic, psychrophilic, halophilic, alkalophilic, and an faculative anaerobe? (5 points)

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4. Use the following data from a bacterial fermentation to calculate the following parameters (25 points). Plot the data on the following page.

A. What is the approximate length of time of the lag phase?

B. Over what time period does the culture exhibit Log phase growth?

C. What is the doubling time of the bacteria in minutes? Is this "fast growth" or "slow growth"?

D. What is the approximate A₆₀₀ of the stationary phase? At what time does the culture achieve 1/2 stationary phase density?

E. Given the doubling time, and assuming the culture is grown at 37°C, what can you infer about the media?

5. Ampicillin and tetracycline are both anti-biotics. However, they work quite differently. Explain how these anti-biotics work, and how they are different from each other. Explain how the ampicillin and tetracycline resistance genes work. (15 points)

6. Define the following terms (10 points):

A. Gene

B. Promoter

C. Operon

D. Operator

E. Polycistronic

7. (10 points) Given the following information, fill in the blanks in the table

8. (15 points) Given the following tRNA molecules indicate the associated amino acid (give either the single or 3 letter acronym for the amino acid)